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KMID : 1098420130210040301
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Korean Journal of Medicinal Crop Science
2013 Volume.21 No. 4 p.301 ~ p.305
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α-Glucosidase Inhibition Activity of Methanol Extracts and Fractions Obtained from Three Dryopteridaceae Species
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Kim Na-Rae
Chi Lai-Won
Lee Chul-Hee
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Abstract
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This study was performed to select adequate plant materials for developing a natural α-glucosidase inhibitor by analyzing α-glucosidase inhibition activity in fronds and rhizomes of three Dryopteridaceae species: Cyrtomium fortunei, Polystichum polyblepharum, and P. lepidocaulon. The highest α-glucosidase inhibitor obtained from frond of P. lepidocaulon (4.16 ㎍ ? mL-1), and rhizome of C. fortunei (1.84 ㎍ ? mL-1), showed much higher inhibition activity than acarbose (1413.70 ㎍ ? mL-1). The biomass required to inhibit α-glucosidase by 50% was 0.04 ~ 0.35 ㎎ for frond and 0.03 ~ 0.10 ㎎ for rhizome, and P. lepidocaulon required the least amount of fronds and P. lepidocaulon the least rhizomes. In frond, α-glucosidase inhibition activity was the highest in water fraction of C. fortunei (20.2 ㎍ ? mL-1), and n-butanol fraction of P. lepidocaulon (9.33 ㎍ ? mL-1) and P. polyblepharum (5.10 ㎍ ? mL-1). In rhizome, it was the highest in n-butanol fractions of C. fortunei (19.76 ㎍ ? mL-1) and P. polyblepharum (4.47 ㎍ ? mL-1), and ethylacetate fraction of P. lepidocaulon (5.46 ㎍ ? mL-1). The frond biomass required for 50% α-glucosidase inhibition was the lowest in the water fraction of C. fortunei (1.43 ㎎), and n-butanol fractions of P. lepidocaulon (1.10 ㎎) and P. polyblepharum (0.66 ㎎). The required biomass of rhizome was the lowest in the water fraction of C. fortunei (1.59 ㎎), and n-hexane fractions of P. lepidocaulon (0.04 ㎎) and P. polyblepharum (0.15 ㎎). The result of this study suggested that the three Dryopteridaceae species had high α-glucosidase inhibition activity with small biomass, which might have high value as materials for economical anti-diabetic medication.
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KEYWORD
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Cyrtomium fortunei, Polystichum lepidocaulon, Polystichum polyblepharum, IC50 Value, Required Fresh Material
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